Development of an enzyme-linked immunosorbent assay kit for Mycoplasma hyorhinis
JS Wu, KM Huang, CS Chang, JH Lin, JP Wang, ZW Chen, CW Hsu
Mycoplasma hyorhinis is a common pathogen in pig farms. Pigs infected by M. hyorhinis exhibit polyserositis and arthritis, and demonstrate reduced growth and lower feed conversion ratios, causing economic loss in pig units. At present, an infection of M. hyorhinis can be detected using polymerase chain reaction (PCR) and indirect hemagglutination (IHA) methods. However, PCR methods cannot be used to detect large numbers of samples at the same time, and the IHA method is complicated and interpretation of the results is not easy. Until now there has not been a commercial ELISA kit for detection of M. hyorhinis and for this reason an anti-M. hyorhinis antibody ELISA kit was developed using an innovative method of antigen preparation. The ELISA kit demonstrated both high sensitivity and specificity, giving a high correlation with the IHA method. Having demonstrated equivalence therefore, this ELISA kit can be used for vaccine research and disease diagnosis and monitoring.