The 2016 Symposium of Animal Vaccine Development Center

Antigen screening for the Actinobacillus pleuropneumoniae subunit vaccine

ZY Yang, JC Hung, HH Wu, WC Huang, YL Zhuo, JH Lin, JP Wang, ZW Chen, CW Hsu

Actinobacillus pleuropneumoniae (APP) is a pathogen of swine respiratory tract illness, which causes high morbidity and high mortality due to lung bleeding, abscess and polyserositis, usually found in finishing pigs. The clinical signs of APP infection include fever, breathing distress, cyanosis or sudden death. Pigs chronically infected with APP showed retarded growth, which causing serious economic losses. In this study, the in-house made ELISA kit was used to evaluate the sensitivities of different APP serotypes in the porcine sera, and single antigen subunit vaccines were immunized in mice to evaluate the efficacy against different APP serotypes. Total of 19 single antigen subunit vaccines were produced by reverse vaccinology technique. The results showed that in-house made ELISA kit recognized different APP serotypes in the porcine sera, and several single antigen subunit vaccines showed good efficacy against challenge of different APP serotypes. The combinations of different antigen subunit vaccines will be tested the efficacy in the pigs.