The 2014 Conference of Chinese Society of Veterinary Science

Development of an enzyme-linked immunosorbent assay kit for Mycoplasma hyopneumoniae

JS Wu, CW Hsu, KM Huang, CS Chang, JH Lin, JP Wang, ZW Chen

Mycoplasma hyopneumoniae is the pathogen causing swine enzootic pneumonia (SEP). Pigs infected by M. hyopneumoniae exhibit reduced growth and lower feed conversion ratios causing economic loss in pig units. Enzyme-linked immunosorbent assay (ELISA) and indirect hemagglutination (IHA) can both detect infection with M. hyopneumoniae. However, the IHA method is complicated and interpretation of results is not easy, whereas commercial ELISA kits are easy to use but expensive. A low cost ELISA kit for the detection of anti-M. hyopneumoniae antibody with high specificity and sensitivity was developed using an innovative method of antigen preparation. The ELISA kit demonstrated a high correlation with the IHA method enabling this kit to be used in place of the IHA method for anti-M. hyopneumoniae antibody detection.